In the following, the online help texts for all data entry fields provided with any Endpoint study record for this IUCLID section are listed. For sections 4 to 10, these guidance notes are completely based on the so-called OECD Harmonised Templates (see Rationale behind IUCLID Endpoint Study Records - OECD harmonised templates in chapter chapter D.4.7.1 What is an Endpoint study record?)
IUCLID per se does not prescribe how detailed the study summaries should be recorded. Refer to the relevant guidance for the respective chemical regulatory programme thereof.
For technical guidance on how to manage Endpoint study records, see chapter D.4.7 How to manage Endpoint study records in sections 4 - 13. For details on data types, see chapter D.4.5 What data types are available for input fields and how are they used?
Under this main heading, fields are subsumed for identifying the purpose of the record (e.g., "key study"), the type of result (e.g., "experimental study"), data waiving indication (if any), reliability indication, and flags for indicating the regulatory purpose envisaged and/or any confidentiality restrictions. This kind of data characterise the relevance of a study summary and are therefore displayed on top of each Endpoint Study Record. For detailed guidance, refer to chapter D.4.7.7.1 Administrative data.
Please note: Data collection method is the analytical method used to collect quantitative residue data by analysing the analyte(s) in the matrices. This method can be identical with or differ from the so-called enforcement method which has to be recorded under the heading "Enforcement method (if applicable)". Enforcement method is a validated analytical method which can be applied by the regulatory agency for enforcing the proposed tolerance, i.e. maximum residue limits (MRL) for pesticides.
In other than residue analyses, the method and results should be described in the fields for Data collection method. Fields being not applicable can be ignored.
If a confirmatory method was used (i.e. applying techniques to demonstrate specificity in case the original method is not highly specific), indicate the instrument / detector used and other relevant details.
Use this field to include any background information, if required, or any relevant introductory remarks on the study summary. Leave field empty if not applicable. Do not include information for which specific fields are provided. For instance, include any background information on the test substance in fields on "Test materials".
PURPOSE OF THIS TEMPLATE:
This template can be used for summarising analytical methods for determing a given substance in various matrices. Depending on the requirements of the relevant legislation, methods for the following matrices may have to be recorded: soil, sediment, suspended particulates, air, water (including drinking water), animal and human body fluids and tissues, plants, plant products, food and feedingstuffs, formulated product, other.
Indicate the bibliographic reference of the study report or publication the study summary is based on. Always enter the primary reference in the first block of fields (i.e. Sort no. = 1), if there are more than one reference to be cited. Copy this block of fields for specifying any other references related to this record (e.g. report of a preliminary study or other documentation). If results of a study report have been published, indicate the full citation of that publication(s) in addition to the reference of the original study.
Table E.519. Field Descriptions
| Reference type | Indicate the type of reference, e.g. "Study report" or "Publication". Select "Other company data" to characterise any unpublished information from a company other than a study report. Select "Grey literature" for any other unpublished information or "other:" and specify. |
| Author(s) (or transferred reference) (Author) | For ease of sorting and searchability use following convention: Surname, Initial (Example 1: White D, Ruehl KJ, Borman SA & Little J. Example 2: Hartley M & Murray W (avoid unnecessary full-stops, commas)). If no individuals are cited as authors, enter name of company or organisation or "Anon." as appropriate. Note that the complete bibliographic reference may appear in this field after migration of unstructured data from existing databases. |
| Year | Enter year of study report or publication. For a study report this field should be completed to include it in any searches, regardless of whether the complete date is given in field "Report date". |
| Title | Include the title of the report. For publications, include the title of the article of a journal or article/chapter of a book (e.g. handbook). |
| Bibliographic source | Not relevant for any study report. For publications or any other literature source (grey literature) specify the following type of information: (i) Title of scientific journal or book (e.g. if handbook); (ii) Volume of journal; (iii) Editor, publisher, place of publication for books or articles in books; (iv) Pagination. Example 1 (journal): J. Agric. Food Chem. 38: 215-227 Example 2 (handbook): In: Lyman WJ (ed.) Handbook of chemical property estimation methods. Environmental behavior of organic compounds. McGraw-Hill Book Company 15.1-15.34, New York. |
| Testing laboratory | Either manually enter the name of the testing laboratory or select it from the picklist. In either case, editing is possible. |
| Report no. | Specify the report number allocated by the testing laboratory. Note that any company-specific study number should be included in the respective field. |
| Owner company | Either manually enter the identity of the company who owns the data or select it from the picklist. In either case, editing is possible. |
| Company study no. | Specify any company study no. if there is such a number and if it is different from the report no. of the testing laboratory. Otherwise leave field empty. |
| Report date | Specify the complete date of the study report, e.g. "2005-05-12" for 12 May 2005. Note that subfield "Year" should be completed in any case for sorting and searching purposes. |
Select appropriate indication for data access. Enter "Not applicable" if the summary consists of information that is commonly accessible such as guidance on safe use.
Indicate as appropriate. Note: "yes" should be selected only if "Data submitter is data owner" or "Data submitter has Letter of Access". Options "yes, but willing to share" or "yes, but not willing to share" may be relevant for specific regulatory programmes where the submitter is requested to indicate whether he is willing to share studies (e.g. with vertebrates).
In the supplementary remarks field, include an explanation as appropriate, i.e. justification for denial of sharing the corresponding study or refer to a document attached that provides justification (e.g. "for justification see attached document X")
A cross-reference can be included to indicate that the same study is recorded in another record. Indicate the respective chapter and record ID and enter relevant explanatory text. This may be useful if specific endpoints of a given study are described in another chapter (e.g. results on reproduction toxicity in case of a combined repeated dose / reproduction toxicity study) or if more than one experiment is described by the same study report, but included in separate records.
Check with the relevant guidance document whether all the methodology details must be repeated or whether a cross-reference to the same study in another chapter may suffice.
Note that any such cross-reference may become useless if a record is either printed or exchanged on its own.
Indicate the medium for which the analytical method is described. In the supplementary remarks field, you can add explanations as appropriate.
Note: The picklist is not descriptive as to whether analytical methods have to be submitted for each of the matrices provided in the picklist. Consult with the programme-specific guidance (e.g. EU BPD, OECD HPVC, Pesticides NAFTA or EU REACH) as to what matrices need to be addressed. If the methods for several matrices can be summarised in one record, you can copy this field for indicating the respective matrices.
Indicate according to which test guideline the study was conducted. If no test guideline was explicitly followed, but the methodology used is equivalent or similar to a specific guideline, you can indicate so in the "Qualifier" subfield preceding the field "Guideline".
Copy this block of fields for specifying more than one guideline (e.g. US EPA in addition to OECD guideline).
Table E.520. Field Descriptions
| Qualifier | Select appropriate qualifier, i.e. - "according to" (if a given test guideline was followed); - "equivalent or similar to" (if no test guideline was explicitly followed, but the methodology is equivalent or similar to a specific guideline); - "no guideline followed" (if none of above qualifiers apply. If so, fill in field "Principles of method if other than guideline"); - "no guideline available" (if so, fill in field "Principles of method if other than guideline"). - "no guideline required" (if so, fill in field "Principles of method if other than guideline"). |
| Guideline | Select the applicable test guideline, e.g. "OECD Guideline xxx". If the test guideline used is not listed, choose "other guideline:" and specify the test guideline in the related text field. In this text field, you can also enter any remarks as applicable, particularly: - To include any other title of the test guideline draft used, a subtitle, another version or update number and the year of update (For instance, different titles and/or numbers may exist for a given EU test guideline.); - To indicate if a the study was performed prior to the adoption of the test guideline specified; - To indicate if the methodology used was based on an extension of the test guideline specified. |
| Deviations from guideline (Deviations) | For robust study summaries or as requested by the regulatory programme, indicate if there are any deviations from the test guideline specified. If "yes" is selected, only briefly state relevant deviations in the supplementary remarks field (e.g. "other species used"); details should be described in the respective fields of the section MATERIALS AND METHODS. |
If no guideline was followed, include a description of the principles of the test protocol or estimated method used in the study. Details should be entered in appropriate distinct fields of section MATERIALS AND METHODS if available. Also provide a justification for using this method if appropriate.
If an estimation method was used (to be indicated in field "Test result type") state the equation(s) and/or computer software or other methods applied to calculate the value(s).
Indicate whether the study was conducted following Good Laboratory Practice or not. Select "yes (incl. certificate)" if a GLP certificate of a test facility is available. Select "yes" if a GLP compliance statement is available, but no information on a GLP certificate. You can give an explanation in the supplementary remarks field, e.g. for explaining why GLP was not complied with or for specifying which (national) GLP was followed.
Indicate if the test material used in the study is equivalent to the submission substance identity. If "yes" is selected, the corresponding identity is automatically entered in the subsequent block of fields "Test material identity".
If "no" is selected, identify the test material in the subsequent block of fields "Test material identity". In this case, also make sure that the information entered in field "Study result type" is consistent, i.e. "read-across from supporting substance (structural analogue or surrogate)".
NOTE: If a completed record is used for another submission, you may have to update both fields "Study result type" and "Test material equivalent to submission substance identity".
If the identity of the test material used for this study is not included in this block of fields automatically, indicate the identity for one or more appropriate identifiers, e.g. CAS number, CAS name, IUPAC name. Copy this block of fields as appropriate.
If another than the submission substance identity was selected erraneously, go back to field "Test material equivalent to submission substance identity" and select "yes". This will prompt automatic entry of the respective identifiers.
Table E.521. Field Descriptions
| Identifier | Select an appropriate identifier from drop-down list, e.g. "CAS number". Use "Other:" and specify, if identity according to a standard identifier is not known or if an additional chemical name or number is provided. |
| Identity | Select the corresponding substance identity from drop-down list or enter manually if the identity is not available from the list or if no list is provided for the type of identifier selected. |
Use freetext template and delete/add elements as appropriate. Enter any details that could be relevant for evaluating this study summary or that are requested by the respective regulatory programme. Consult the programme-specific guidance (e.g. OECD HPVC, Pesticides NAFTA or EU REACH) thereof.
Note that any information that can be claimed confidential should be included in the subsequent field "Confidential details on test material".
Explanations:
- Name of test material (as cited in study report): only if different from any other identifiers provided in the preceding fields.
- Molecular formula (if other than submission substance): specify
- Molecular weight (if other than submission substance): specify
- Smiles notation (if other than submission substance): provide if available
- InChl (if other than submission substance): provide if available
- Structural formula attached as image file (if other than submission substance): see Fig.: only if different from submission substance. Indicate Fig. no. if a file is attached in field "Attached document", e.g. state "see Fig. 1".
- Substance type: indicate whether pure active substance, technical product, formulation or other.
- Physical state: indicate "gas", "solid" or "liquid" only if different from submission substance or if substance can occur in different physical states.
- Analytical purity: specify in %
- Impurities (identity and concentrations): specify
- Composition of the test material, percentage of components: specify if applicable
- Isomers composition: specify if applicable
- Purity test date: provide if available
- Lot/batch No.: provide if available
- Expiration date of the lot/batch: provide if available
- Radiochemical purity (if radiolabelling): specify if applicable
- Specific activity (if radiolabelling): specify if applicable
- Locations of the label (if radiolabelling): specify if applicable
- Expiration date of radiochemical substance (if radiolabelling): specify if applicable
- Storage condition of test substance: specify if applicable
- Stability under test conditions: indicate if available
Enter any confidential information on the test material in this separate field. Use freetext template and delete/add elements as appropriate. Enter any details that could be relevant for evaluating this study summary or that are requested by the respective regulatory programme. Consult the programme-specific guidance (e.g. OECD HPVC, Pesticides NAFTA or EU REACH) thereof.
Explanations:
- Analytical purity: specify in %
- Impurities (identity and concentrations): specify
- Composition of the test material, percentage of components: specify if applicable
- Purity test date: provide if available
- Lot/batch No.: : provide if available
- Expiration date of the lot/batch: : provide if available
- Isomers composition: specify if applicable
ONLY if the study summary is a read-across to another substance, i.e. analogue or surrogate (e.g. degradation / transformation product), enter any relevant details on the physico-chemical or other relevant properties. Use freetext template and delete/add elements as appropriate.
Note that the physico-chemical properties of the substance for which the submission is made should be recorded in the corresponding chapters (templates) and therefore need not be repeated here. Nevertheless, the possible influence of any physico-chemical properties should be indicated / discussed in appropriate fields, particularly in fields "Details on test conditions" and "Details on results". This holds also true if any property of the substance is different in the test medium as compared to the data recorded in the section on physico-chemical properties, e.g. lower water solubility.
Indicate the instrument / detector used for the quantitative analysis of the parent compound / transformation products including the type of detector, e.g. "HPLC-UV". Copy this field if more than one method needs to be specified.
Give any further details in field "Details on data collection method".
Briefly describe further details on the principles of the method used to detect the analytes (to be specified, e.g. "parent compound", "parent and transformation products" or "transformation product: .....") in matrices. Use freetext template and delete/add elements as appropriate. As an option you may include an excerpt from the study report.
Enter any details that could be relevant for evaluating this study summary or that are requested by the respective regulatory programme. Consult the programme-specific guidance (e.g. OECD HPVC, Pesticides NAFTA or EU REACH) thereof. If further details are required, insert (sub)headings) as appropriate, e.g.:
INSTRUMENTATION
- Make(s)/model(s) of instrumentation used:
- Specifity of detector(s):
- Columns (packing materisl, size):
- Carrier gases: for GC:
- Operating procedures:
- Mobile phase for HPLC:
- Retention times for substance and standards:
CHROMATOGRAMS: see Fig. attached
INTERFERING SUBSTANCE(S):
STABILITY OF PARENT AND TRANSFORMATION PRODUCTS AT VARIOUS STAGES OF ANALYSIS:
PROBLEMS / PRECAUTIONS
- Special problems encountered:
- Precautions to be taken during
- analysis of samples:
- handling of samples:
- storage of samples:
TOTAL TIME FOR COMPLETION:
Indicate the instrument / detector used in the enforcement method. If applicable, select "other:" and state "enforcement same as data collection method".
Copy this field if more than one method needs to be specified.
Give any further details in field "Details on data enforcement method".
Briefly describe further details on the principles of the enforcement method if applicable (otherwise leave field empty). Use freetext template and delete/add elements as appropriate. As an option you may include an excerpt from the study report.
Enter any details that could be relevant for evaluating this study summary or that are requested by the respective regulatory programme. Consult the programme-specific guidance (e.g. OECD HPVC, Pesticides NAFTA or EU REACH) thereof. If further details are required, insert (sub)headings) as appropriate, e.g.:
INSTRUMENTATION
- Make(s)/model(s) of instrumentation used:
- Specifity of detector(s):
- Columns (packing materisl, size):
- Carrier gases: for GC:
- Operating procedures:
- Mobile phase for HPLC:
- Retention times for substance and standards:
CHROMATOGRAMS: see Fig. attached
INTERFERING SUBSTANCE(S):
STABILITY OF PARENT AND TRANSFORMATION PRODUCTS AT VARIOUS STAGES OF ANALYSIS:
PROBLEMS / PRECAUTIONS
- Special problems encountered:
- Precautions to be taken during
- analysis of samples:
- handling of samples:
- storage of samples:
TOTAL TIME FOR COMPLETION:
Indicate the instrument / detector used in the confirmatory method (if any). If applicable, select "other:" and state "no confirmatory method used/required".
Copy this field if more than one method needs to be specified.
Give any further details in field "Details on data confirmatory method".
Briefly describe further details on the principles of the confirmatory method if any.
Enter any details that could be relevant for evaluating this study summary or that are requested by the respective regulatory programme. Consult the programme-specific guidance (e.g. OECD HPVC, Pesticides NAFTA or EU REACH) thereof.
In this field, you can enter any information on materials and methods, for which no distinct field is available, or transfer free text from other databases. You can also open a rich text editor and create formatted text and tables or insert and edit any excerpt from a word processing or spreadsheet document, provided it was converted to the HTML format.
Note: One rich text editor field each is provided for the MATERIALS AND METHODS and RESULTS section. In addition the fields "Overall remarks" and "Executive summary" allow rich text entry.
Indicate the compound (analyte) addressed (e.g. "parent compound", "parent and transformation products" or "transformation product: ....."). Report the recovery rates at each level (e.g. at spiking level 1, spiking level 2 etc.) at the limit of quantification and give the mean % recovery and the relative standard deviation in % including the number of recovery studies. Include a brief evaluation of the repeatability of the method (e.g. "These values demonstrate that the method has satisfactory repeatability.")
Use freetext template and delete/add elements as appropriate, or include a table (particularly for comprehensive data) in rich text field "Any other information on results incl. tables". Upload predefined table(s) if any or adapt table(s) from study report. Use table numbers in the sequence in which you refer to them in the text (e.g. "... see Table 1").
Note: Specific tables may be required. Consult the programme-specific guidance (e.g. OECD HPVC, Pesticides NAFTA or EU REACH) thereof.
For each compound (analyte) addressed (to be specified, e.g. "parent compound", "parent and transformation products" or "transformation product: ....."), report both the limit of quantitation (LOQ) and limit of detection (LOD), and the criteria used to determine the LOD or LOQ, i.e., the lowest fortification/spiking level or S/N ratio.
Use freetext template, delete/add elements and edit text set in [...] as appropriate, or include a table (particularly for comprehensive data) in rich text field "Any other information on results incl. tables". Upload predefined table(s) if any or adapt table(s) from study report. Use table numbers in the sequence in which you refer to them in the text (e.g. "... see Table 1").
Note: Specific tables may be required. Consult the programme-specific guidance (e.g. OECD HPVC, Pesticides NAFTA or EU REACH) thereof.
Indicate the compound (analyte) addressed (e.g. "parent compound", "parent and transformation products" or "transformation product: ....."). Report the recovery rates at each level (e.g. at spiking level 1, spiking level 2 etc.) at the limit of quantification and give the mean % recovery and the relative standard deviation in % including the number of recovery studies. Include a brief evaluation of the repeatability of the method (e.g. "These values demonstrate that the method has satisfactory repeatability.")
Use freetext template and delete/add elements as appropriate, or include a table (particularly for comprehensive data) in rich text field "Any other information on results incl. tables". Upload predefined table(s) if any or adapt table(s) from study report. Use table numbers in the sequence in which you refer to them in the text (e.g. "... see Table 1").
Note: Specific tables may be required. Consult the programme-specific guidance (e.g. OECD HPVC, Pesticides NAFTA or EU REACH) thereof.
For each compound (analyte) addressed (to be specified, e.g. "parent compound", "parent and transformation products" or "transformation product: ....."), report both the limit of quantitation (LOQ) and limit of detection (LOD), and the criteria used to determine the LOD or LOQ, i.e., the lowest fortification/spiking level or S/N ratio.
Use freetext template, delete/add elements and edit text set in [...] as appropriate, or include a table (particularly for comprehensive data) in rich text field "Any other information on results incl. tables". Upload predefined table(s) if any or adapt table(s) from study report. Use table numbers in the sequence in which you refer to them in the text (e.g. "... see Table 1").
Note: Specific tables may be required. Consult the programme-specific guidance (e.g. OECD HPVC, Pesticides NAFTA or EU REACH) thereof.
Discuss the independent laboratory validation (ILV) in terms of whether or not it was conducted according to guideline specifications. Discuss any method modifications that may impact the analyses of the residues (e.g., altered LOQ) that are suggested by the independent laboratory. Alternatively, include a table (particularly for comprehensive data) in rich text field "Any other information on results incl. tables". Upload predefined table(s) if any or adapt table(s) from study report. Use table numbers in the sequence in which you refer to them in the text (e.g. "... see Table 1").
Note: Specific tables may be required. Consult the programme-specific guidance (e.g. OECD HPVC, Pesticides NAFTA or EU REACH) thereof.
In this field, you can enter any other remarks on results. You can also open a rich text editor and create formatted text and tables or insert and edit any excerpt from a word processing or spreadsheet document, provided it was converted to the HTML format.
Note: Both the "Materials and methods" section and "Results" section. In addition the fields "Overall remarks" and "Executive summary" allow rich text entry.
In this field, you can enter any overall remarks or transfer free text from other databases. You can also open a rich text editor and create formatted text and tables or insert and edit any excerpt from a word processing or spreadsheet document, provided it was converted to the HTML format.
Note: One rich text editor field each is provided for the MATERIALS AND METHODS and RESULTS section. In addition the fields "Overall remarks" and "Executive summary" allow rich text entry.
Attach any background document that cannot be inserted in any rich text editor field, particularly image files (e.g. an image of a structural formula).
Copy this block of fields for attaching more than one file.
Table E.522. Field Descriptions
| Attached document | Upload file by clicking the upload icon. As appropriate, enter any additional information, e.g. language. The file name is displayed after uploading the document. |
| Remarks | As appropriate, include remarks, e.g. a short description of the content of the attached document if the file name is not self-explanatory. |
If required, an electronic copy of the full study report can be attached as WORD, pdf or other document type, which will not be integrated in any report, but must be handled as separate files.
Note: In the export administration you can indicate whether the attached files should be included in the data export or not.
If required by the respective national/regional programme, briefly summarise the relevant aspects of the study including the conclusions reached. If a specific format is prescribed, upload the respective freetext template if available from the drop-down list or copy it from the corresponding document.
Consult the programme-specific guidance (e.g. OECD HPVC, Pesticides NAFTA or EU REACH) thereof.
A Cross-reference to other study or other studies can be included which are considered relevant in the interpretation of the test results, e.g. for supporting the conclusion that an effect observed was not substance-related. Indicate the respective chapter(s) and record ID(s) and enter relevant explanatory text.
Such cross-references may be useful if it is considered relevant to discuss other results at the summary level of a single study. It should be noted that the overall appraisal of results from different studies is normally done in the hazard or risk assessment.
Note that any such cross-reference may become useless if a record is either printed or exchanged on its own.